Stored RBC metabolism as a function of caffeine levels
D'Alessandro A, Fu X, Reisz JA, Kanias T, Page GP, Stone M, Kleinman S, Zimring JC, Busch M, Recipient Epidemiology and Donor Evaluation Study-III (REDS III)
Transfusion · 31 citas
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Cómo se estudió
- Diseño
- Ensayo clínico controlado (indexado por PubMed)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institutes of Health
- Gobierno
- National Heart, Lung, and Blood Institute
- Gobierno
- National Institute of General Medical Sciences
- Gobierno
- NHLBI NIH HHS
- Gobierno
- NIGMS NIH HHS
- Gobierno
- NIH HHS
- Subvenciones
- National Heart, Lung, and Blood Institute (R01HL146442); National Institute of General Medical Sciences (RM1 GM131968); National Institutes of Health (1s10od021641-01a1); National Heart, Lung, and Blood Institute (R01HL148151); National Heart, Lung, and Blood Institute (RM1GM131968); National Heart, Lung, and Blood Institute (R21 HL150032); National Heart, Lung, and Blood Institute (R01 HL149714); National Heart, Lung, and Blood Institute (HHSN2682011); National Heart, Lung, and Blood Institute (S10OD021641)
Según 6 financiadores declarados.
Publicación
- Publicado
- 2020-05-11 · Transfusion · vol. 60 · número 6 · págs. 1197–1211
- Editorial
- Wiley
- Citado
- 31 citas · más que el 92% de estudios similares · 3.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 76 trabajos
- Acceso
- Acceso abierto (copia en repositorio)
- Áreas de investigación
- Coffee research and impacts · Forensic Toxicology and Drug Analysis · Erythrocyte Function and Pathophysiology
- Palabras clave
- Caffeine, Function (biology), Metabolism, Chemistry, Biochemistry, Medicine, Biology, Internal medicine, Cell biology
- MeSH
- erythrocytes, humans, caffeine, xanthine, blood preservation, glycolysis, coffee, adult, female, male, pentose phosphate pathway, metabolomics
10 autores
- Angelo D’Alessandro🇺🇸 · autor de correspondenciaVitalant; Vitalant Research Institute; University of Colorado Denver
- Xiaoyun Fu🇺🇸Bloodworks Northwest
- Julie A. Reisz🇺🇸University of Colorado Denver
- Tamir Kanias🇺🇸Vitalant; Vitalant Research Institute; University of Colorado Denver
- Grier P. Page🇺🇸RTI International
- Mars Stone🇺🇸Pacific Research Institute; Vitalant; Vitalant Research Institute
- Steve H. Kleinman🇨🇦University of British Columbia
- James C. Zimring🇺🇸University of Virginia
- Michael Paul Busch🇺🇸Pacific Research Institute; Vitalant; Vitalant Research Institute
- for the Recipient Epidemiology and Donor Evaluation Study‐III (REDS III)
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Coffee consumption is extremely common in the United States. Coffee is rich with caffeine, a psychoactive, purinergic antagonist of adenosine receptors, which regulate red blood cell energy and redox metabolism. Since red blood cell (purine) metabolism is a critical component to the red cell storage lesion, here we set out to investigate whether caffeine levels correlated with alterations of energy and redox metabolism in stored red blood cells.
Study design and methods
We measured the levels of caffeine and its main metabolites in 599 samples from the REDS-III RBC-Omics (Recipient Epidemiology Donor Evaluation Study III Red Blood Cell-Omics) study via ultra-high-pressure-liquid chromatography coupled to high-resolution mass spectrometry and correlated them to global metabolomic and lipidomic analyses of RBCs stored for 10, 23, and 42 days.
Results
Caffeine levels positively correlated with increased levels of the main red cell antioxidant, glutathione, and its metabolic intermediates in glutathione-dependent detoxification pathways of oxidized lipids and sugar aldehydes. Caffeine levels were positively correlated with transamination products and substrates, tryptophan, and indole metabolites. Expectedly, since caffeine and its metabolites belong to the family of xanthine purines, all xanthine metabolites were significantly increased in the subjects with the highest levels of caffeine. However, high-energy phosphate compounds ATP and DPG were not affected by caffeine levels, despite decreases in glucose oxidation products-both via glycolysis and the pentose phosphate pathway.
Conclusion
Though preliminary, this study is suggestive of a beneficial correlation between the caffeine levels and improved antioxidant capacity of stored red cells.
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