Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells
De Robertis M, Sarra A, D'Oria V, Mura F, Bordi F, Postorino P, Fratantonio D
Biomolecules · 146 citas
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- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Financiación no declarada
Publicación
- Publicado
- 2020-05-10 · Biomolecules · vol. 10 · número 5 · pág. 742
- Editorial
- Multidisciplinary Digital Publishing Institute
- Citado
- 187 citas · más que el 97% de estudios similares · 5.5× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 52 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Extracellular vesicles in disease · MicroRNA in disease regulation · Circular RNAs in diseases
- Palabras clave
- microRNA, Cell biology, Microvesicles, Biology, KEGG, Gene, Gene expression, Exosome, Transcriptome, Biochemistry
- MeSH
- cell line, humans, inflammation, reactive oxygen species, tumor necrosis factor-alpha, micrornas, rna, messenger, cell death, cell survival, endocytosis, gene expression regulation, base sequence, oxidative stress, cytoprotection, nanoparticles, exosomes, gene ontology, blueberry plants
7 autores
- Mariangela De Robertis🇮🇹Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies; National Research Council; University of Bari Aldo Moro
- Angelo Sarra🇮🇹Roma Tre University; Sapienza University of Rome
- Valentina Doria🇮🇹Bambino Gesù Children's Hospital; Istituti di Ricovero e Cura a Carattere Scientifico
- Francesco Mura🇮🇹Sapienza University of Rome
- F. Bordi🇮🇹Sapienza University of Rome
- P. Postorino🇮🇹Sapienza University of Rome
- Deborah Fratantonio🇮🇹 · autor de correspondenciaBambino Gesù Children's Hospital; Istituti di Ricovero e Cura a Carattere Scientifico; University of Bari Aldo Moro
Resumen
El texto original del estudio, en el idioma en que se publicó.
Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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