The effect of dietary nitrate supplementation on the speed-duration relationship in mice with sickle cell disease
Ferguson SK, Redinius KM, Harral JW, Pak DI, Swindle DC, Hirai DM, Blackwell JR, Jones AM, Stenmark KR, Buehler PW, Irwin DC
Journal of applied physiology (Bethesda, Md. : 1985) · 16 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institutes of Health
- Gobierno
- National Heart, Lung, and Blood Institute
- Gobierno
- National Institute of General Medical Sciences
- Gobierno
- National Institute of Diabetes and Digestive and Kidney Diseases
- Gobierno
- NIGMS NIH HHS
- Universidad u hospital
- Colorado Nutrition and Obesity Research Center
- Gobierno
- NIH HHS
- Gobierno
- HHS | National Institutes of Health
- Gobierno
- NHLBI NIH HHS
- Gobierno
- NIDDK NIH HHS
- Subvenciones
- National Heart, Lung, and Blood Institute (P01-HL-014985); National Institute of Diabetes and Digestive and Kidney Diseases (P30 DK048520); National Heart, Lung, and Blood Institute (T32HL007171); National Institute of General Medical Sciences (P20GM 103466); National Heart, Lung, and Blood Institute (R01 HL125642); National Institutes of Health (1r01hl125642-01a1); National Institutes of Health (DK048520); National Institutes of Health (P30DK048520); National Institutes of Health (T32-Hl007171)
Según 10 financiadores declarados.
Publicación
- Publicado
- 2020-07-23 · J Appl Physiol (1985) · vol. 129 · número 3 · págs. 474–482
- Editorial
- American Physiological Society
- Citado
- 17 citas · más que el 75% de estudios similares · 1.0× el promedio de su campo
- Referencias
- 65 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Hemoglobinopathies and Related Disorders · Iron Metabolism and Disorders · Peroxisome Proliferator-Activated Receptors
- Palabras clave
- Disease, Medicine, Dietary Nitrate, Sickle cell anemia, Internal medicine, Cell, Endocrinology, Nitrate, Treadmill, Anemia, Physiology, Biology, Nitrite, Biochemistry
- MeSH
- animals, humans, mice, beta vulgaris, anemia, sickle cell, nitrates, double-blind method, oxygen consumption, exercise tolerance, dietary supplements
11 autores
- Scott K. Ferguson🇺🇸 · autor de correspondenciaUniversity of Hawaii at Hilo; University of Colorado Anschutz; University of Colorado Denver
- Katherine M. Redinius🇺🇸University of Colorado Anschutz; University of Colorado Denver
- Julie Wright Harral🇺🇸University of Colorado Anschutz; University of Colorado Denver
- David I. Pak🇺🇸University of Colorado Anschutz; University of Colorado Denver
- Delaney C. Swindle🇺🇸University of Colorado Anschutz; University of Colorado Denver
- Daniel M. Hirai🇺🇸Purdue University West Lafayette
- Jamie R. Blackwell🇬🇧University of Exeter
- ANDREW MARK JONES🇬🇧University of Exeter
- Kurt R. Stenmark🇺🇸University of Colorado Anschutz; University of Colorado Denver
- Paul W. Buehler🇺🇸University of Maryland, Baltimore
- David C. Irwin🇺🇸University of Colorado Anschutz; University of Colorado Denver
Resumen
El texto original del estudio, en el idioma en que se publicó.
Sickle cell disease (SCD) causes exercise intolerance likely due to impaired skeletal muscle function and low nitric oxide (NO) bioavailability. Dietary nitrate improves hemodynamic and metabolic control during exercise in humans and animals. The purpose of this investigation was to assess the impact of nitrate supplementation on exercise capacity as measured by the running speed to exercise duration relationship [critical speed (CS)]in mice with SCD. We tested the hypothesis that nitrate supplementation via beetroot juice (BR) would attenuate the exercise intolerance observed in mice with SCD. Ten wild-type (WT) and 18 Berkley sickle-cell mice (BERK) received water (WT: n = 10, BERK: n = 10) or nitrate-rich BR (BERK+BR: n = 8, nitrate dose 1 mmol/kg/day) for 5 days. Following the supplementation period, all mice performed 3-5 constant-speed treadmill tests that resulted in exhaustion within 1.5 to 20 min. Time to exhaustion vs. treadmill speed was fit to a hyperbolic model to determine CS. CS was significantly lower in BERK vs. WT and BERK+BR with no significant difference between WT and BERK+BR (WT: 36.6 ± 1.6, BERK: 23.8 ± 1.5, BERK+BR: 31.1 ± 2.1 m/min, P NEW & NOTEWORTHY Sickle cell disease compromises muscle O2 delivery resulting in exercise intolerance. Dietary nitrate supplementation increases skeletal muscle blood flow during exercise and may improve exercise capacity in a mouse model of sickle cell disease. We investigated the effects of dietary nitrate supplementation on exercise tolerance in a mouse model of sickle cell disease using the treadmill speed-duration relationship (critical speed). Mice with sickle cell disease provided with a dietary nitrate supplement had a critical speed not significantly different from healthy wild-type mice.
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