Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts
Mahajan AS, Arikatla VS, Thyagarajan A, Zhelay T, Sahu RP, Kemp MG, Spandau DF, Travers JB
Nutrients · 13 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- U.S. Department of Veterans Affairs
- Gobierno
- National Institutes of Health
- Universidad u hospital
- School of Medicine, Indiana University
- Gobierno
- National Institute on Aging
- Gobierno
- National Institute of General Medical Sciences
- Gobierno
- NIH HHS
- Gobierno
- Dayton VA Medical Center
- Subvenciones
- U.S. Department of Veterans Affairs (I01 CX002241); National Institute on Aging (R01AG048946); U.S. Department of Veterans Affairs (I01 BX000853); National Institute of General Medical Sciences (R01GM130583); National Institutes of Health (1101CX000809); U.S. Department of Veterans Affairs (I01 CX001904); U.S. Department of Veterans Affairs (I01 CX001956); National Institutes of Health (AG048946); National Institutes of Health (R01 HL062996); National Institutes of Health (HL062996); National Institutes of Health (R01AG048946)
Según 7 financiadores declarados.
Publicación
- Publicado
- 2021-11-16 · Nutrients · vol. 13 · número 11 · pág. 4102
- Editorial
- Multidisciplinary Digital Publishing Institute
- Citado
- 19 citas · más que el 88% de estudios similares · 2.2× el promedio de su campo
- Referencias
- 52 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Skin Protection and Aging · Telomeres, Telomerase, and Senescence · Sirtuins and Resveratrol in Medicine
- Palabras clave
- Senescence, Dermal fibroblast, Cell biology, Fibroblast, Skin Aging, Oxidative stress, Carcinogenesis, Biology, Chemistry, Cancer research, Endocrinology, Biochemistry, In vitro, Medicine
- MeSH
- fibroblasts, dermis, humans, hydrogen peroxide, creatine, niacinamide, insulin-like growth factor i, rna, messenger, oxidants, skin aging, aged, cellular senescence, senescence-associated secretory phenotype
8 autores
- Avinash S. Mahajan🇺🇸Wright State University
- Venkata Sreekanth Arikatla🇺🇸Wright State University
- Anita Thyagarajan🇺🇸Wright State University
- Tetyana Zhelay🇺🇸Wright State University
- Ravi P. Sahu🇺🇸Wright State University
- Michael G. Kemp🇺🇸Wright State University; Dayton VA Medical Center
- Dan F. Spandau🇺🇸Richard L. Roudebush VA Medical Center; Indiana University School of Medicine; Indiana University – Purdue University Indianapolis
- Jeffrey Bryant Travers🇺🇸 · autor de correspondenciaWright State University; Dayton VA Medical Center
Resumen
El texto original del estudio, en el idioma en que se publicó.
Dermal fibroblasts provide structural support by producing collagen and other structural/support proteins beneath the epidermis. Fibroblasts also produce insulin-like growth factor-1 (IGF-1), which binds to the IGF-1 receptors (IGF-1Rs) on keratinocytes to activate signaling pathways that regulate cell proliferation and cellular responses to genotoxic stressors like ultraviolet B radiation. Our group has determined that the lack of IGF-1 expression due to fibroblast senescence in the dermis of geriatric individuals is correlated with an increased incidence of skin cancer. The present studies tested the hypothesis that pro-energetics creatine monohydrate (Cr) and nicotinamide (NAM) can protect normal dermal human fibroblasts (DHF) against experimentally induced senescence. To that end, we used an experimental model of senescence in which primary DHF are treated with hydrogen peroxide (H2O2) in vitro, with senescence measured by staining for beta-galactosidase activity, p21 protein expression, and senescence associated secretory phenotype cytokine mRNA levels. We also determined the effect of H2O2 on IGF-1 mRNA and protein expression. Our studies indicate that pretreatment with Cr or NAM protects DHF from the H2O2-induced cell senescence. Treatment with pro-energetics post-H2O2 had no effect. Moreover, these agents also inhibited reactive oxygen species generation from H2O2 treatment. These studies suggest a potential strategy for protecting fibroblasts in geriatric skin from undergoing stress-induced senescence, which may maintain IGF-1 levels and therefore limit carcinogenesis in epidermal keratinocytes.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.