Photobiomodulation Effects on Periodontal Ligament Stem Cells: A Systematic Review of In Vitro Studies
Mylona V, Anagnostaki E, Chiniforush N, Barikani H, Lynch E, Grootveld M
Current stem cell research & therapy · 19 citas
Etiquetas de revisión
Financiación no declaradaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Revisión sistemática (indexado por PubMed)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2022-05-31 · Curr Stem Cell Res Ther · vol. 19 · número 4 · págs. 544–558
- Editorial
- Bentham Science Publishers
- Citado
- 30 citas · más que el 78% de estudios similares · 1.3× el promedio de su campo
- Referencias
- 97 trabajos
- Acceso
- Acceso abierto (copia en repositorio) · CC-BY-NC-ND
- Áreas de investigación
- Laser Applications in Dentistry and Medicine · Periodontal Regeneration and Treatments · Mesenchymal stem cell research
- Palabras clave
- low-level laser therapy, periodontal ligament stem cells, LED therapy, near-infrared band, osteogenic differentiation, cell proliferation, inflammation reduction, stemness, fluence, regenerative dentistry, stem cell therapy
- MeSH
- stem cells, periodontal ligament, humans, cell proliferation, osteogenesis, low-level light therapy
6 autores
- Valina Mylona🇬🇧 · autor de correspondenciaDe Montfort University
- Eugenia Anagnostaki🇬🇧De Montfort University
- Nasim Chiniforush🇮🇷Tehran University of Medical Sciences
- Hamidreza Barikani🇮🇷Tehran University of Medical Sciences
- Edward J. Lynch🇬🇧De Montfort University
- Martin C. Grootveld🇬🇧De Montfort University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Stem cell therapy has been considered to play a paramount role in the treatment modalities available for regenerative dentistry. The established beneficial effects of photobiomodulation (PBM) at the cellular level have led to the combined use of these two factors (PBM and stem cells). The main goal of this study was firstly to critically appraise the effects of PBM on periodontal ligament stem cells (PDLSCs), and secondly to explore the most effective PBM protocols applied.
Methods
Pubmed, Cochrane, Scopus, Science Direct, and Google Scholar search engines were used to identify experimental in vitro studies in which PBM was applied to cultured PDLSCs. After applying specific keywords, additional filters, and inclusion/exclusion criteria, a preliminary number of 245 articles were narrowed down to 11 in which lasers and LEDs were used within the 630 - 1064 nm wavelength range. Selected articles were further assessed by three independent reviewers for strict compliance with PRISMA guidelines, and a modified Cochrane risk of bias to determine eligibility.
Statistical analysis
The dataset analysed was extracted from the studies with sufficient and clearly presented PBM protocols. Simple univariate regression analysis was performed to explore the significance of contributions of potential quantitative predictor variables toward study outcomes, and a one-way ANOVA model was employed for testing differences between the laser or LED sources of the treatments. The significance level for testing was set at α = 0.05.
Results
The proliferation rate, osteogenic differentiation, and expression of different indicative genes for osteogenesis and inflammation suppression were found to be positively affected by the application of various types of lasers and LEDs. With regard to the PBM protocol, only the wavelength variable appeared to affect the treatment outcome; indeed, the 940 nm wavelength parameter was found not to exert a favourable effect.
Conclusions
Photobiomodulation can enhance the stemness and differentiation capacities of periodontal ligament stem cells. Therefore, for PBM protocols, there remains no consensus amongst the scientific community. Statistical analyses performed here indicated that the employment of a near-infrared (NIR) wavelength of 940 nm may not yield a significant favourable outcome, although those within the 630 - 830 nm range did so. Concerning the fluence, it should not exceed 8 J/cm2 when therapy is applied by LED devices, and 4 J/cm2 when applied by lasers, respectively.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
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