Financiado en parte por Shiseido Co., Ltd.
Magnesium Supplementation Attenuates Ultraviolet-B-Induced Damage Mediated through Elevation of Polyamine Production in Human HaCaT Keratinocytes
Shu S, Kobayashi M, Marunaka K, Yoshino Y, Goto M, Katsuta Y, Ikari A
Cells · 4 citas
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Financiado por la industriaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiado por la industria
- Gobierno
- Japan Society for the Promotion of Science
- Empresa
- Shiseido Co., Ltd.
- Subvenciones
- Japan Society for the Promotion of Science (19H03373)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2022-07-22 · Cells · vol. 11 · número 15 · pág. 2268
- Editorial
- Multidisciplinary Digital Publishing Institute
- Citado
- 9 citas · más que el 63% de estudios similares · 0.6× el promedio de su campo
- Referencias
- 42 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Polyamine Metabolism and Applications · Skin Protection and Aging · Biomedical Research and Pathophysiology
- Palabras clave
- Polyamine, HaCaT, CREB, Downregulation and upregulation, GSK-3, Chemistry, Oxidative stress, Biochemistry, Protein kinase A, GSK3B, Cell biology, Kinase, Molecular biology, Biology, Transcription factor, In vitro
- MeSH
- cell line, keratinocytes, humans, magnesium, polyamines, glycogen synthase kinase 3, mitogen-activated protein kinase kinases, ultraviolet rays, dietary supplements, cyclic amp response element-binding protein
7 autores
- Shokoku Shu🇯🇵Gifu Pharmaceutical University
- Mao Kobayashi🇯🇵Gifu Pharmaceutical University
- Kana Marunaka🇯🇵Gifu Pharmaceutical University
- Yuta Yoshino🇯🇵Gifu Pharmaceutical University
- Makiko Goto🇯🇵Mirai Hospital
- Yuji Katsuta🇯🇵Mirai Hospital
- Akira Ikari🇯🇵 · autor de correspondenciaGifu Pharmaceutical University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Magnesium ions (Mg2+) have favorable effects such as the improvement of barrier function and the reduction of inflammation reaction in inflammatory skin diseases. However, its mechanisms have not been fully understood. Microarray analysis has shown that the gene expressions of polyamine synthases are upregulated by MgCl2 supplementation in human HaCaT keratinocytes. Here, we investigated the mechanism and function of polyamine production. The mRNA and protein levels of polyamine synthases were dose-dependently increased by MgCl2 supplementation, which were inhibited by U0126, a MEK inhibitor; CHIR-99021, a glycogen synthase kinase-3 (GSK3) inhibitor; and Naphthol AS-E, a cyclic AMP-response-element-binding protein (CREB) inhibitor. Similarly, reporter activities of polyamine synthases were suppressed by these inhibitors, suggesting that MEK, GSK3, and CREB are involved in the transcriptional regulation of polyamine synthases. Cell viability was reduced by ultraviolet B (UVB) exposure, which was rescued by MgCl2 supplementation. The UVB-induced elevation of reactive oxygen species was attenuated by MgCl2 supplementation, which was inhibited by cysteamine, a polyamine synthase inhibitor. Our data indicate that the expression levels of polyamine synthases are upregulated by MgCl2 supplementation mediated through the activation of the MEK/GSK3/CREB pathway. MgCl2 supplementation may be useful in reducing the UVB-induced oxidative stress in the skin.
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