Characterization of interactions of dietary cholesterol with the murine and human gut microbiome
Le HH, Lee MT, Besler KR, Comrie JMC, Johnson EL
Nature microbiology · 119 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- U.S. Department of Health and Human Services
- Organización sin fines de lucro
- Canadian Institute for Advanced Research
- Gobierno
- New York State Stem Cell Science
- Gobierno
- National Institutes of Health
- Gobierno
- National Institute of General Medical Sciences
- Gobierno
- NIGMS NIH HHS
- Gobierno
- NIH HHS
- Gobierno
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences
- Subvenciones
- National Institute of General Medical Sciences (R35 GM138281); National Institutes of Health (1s10od018516-01); National Institutes of Health (5r35gm138281-05); U.S. Department of Health and Human Services (R35GM138281)
Según 8 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2022-08-18 · Nat Microbiol · vol. 7 · número 9 · págs. 1390–1403
- Editorial
- Nature Portfolio
- Citado
- 123 citas · más que el 99% de estudios similares · 7.1× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 94 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY
- Áreas de investigación
- Gut microbiota and health · Metabolomics and Mass Spectrometry Studies · Probiotics and Fermented Foods
- Palabras clave
- Microbiome, Biology, Bacteroides thetaiotaomicron, Bacteroides, Metagenomics, Cholesterol, Biochemistry, Genetics, Bacteria, Gene
- MeSH
- animals, humans, mice, bacteroides, cholesterol, sulfotransferases, dietary fats, cholesterol, dietary, microbiota, gastrointestinal microbiome
5 autores
- Henry H. Le🇺🇸Cornell University
- Min-Ting Lee🇺🇸Cornell University
- Kevin R. Besler🇺🇸Cornell University
- Janine M. C. Comrie🇺🇸Cornell University
- Elizabeth L. Johnson🇺🇸 · autor de correspondenciaCornell University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Consumption of dietary lipids, such as cholesterol, modulates the gut microbiome with consequences for host health through the production of microbiome-derived metabolites. Despite the implications for host metabolism, a limited number of specific interactions of the gut microbiome with diet-derived lipids have been characterized. This is partially because obtaining species-level resolution of the responsible taxa can be challenging and additional approaches are needed to identify health-relevant metabolites produced from cholesterol-microbiome interactions. Here we performed bio-orthogonal labelling sort sequence spectrometry, a click chemistry based workflow, to profile cholesterol-specific host-microbe interactions. Mice were exposed to an alkyne-functionalized variant of cholesterol and 16S ribosomal RNA gene amplicon sequencing of faecal samples identified diet-derived cholesterol-interacting microbes from the genera Bacteroides, Bifidobacterium, Enterococcus and Parabacteroides. Shotgun metagenomic analysis provided species-level resolution of diet-derived cholesterol-interacting microbes with enrichment of bile acid-like and sulfotransferase-like activities. Using untargeted metabolomics, we identify that cholesterol is converted to cholesterol sulfate in a Bacteroides-specific manner via the enzyme BT_0416. Mice monocolonized with Bacteroides thetaiotaomicron lacking Bt_0416 showed altered host cholesterol and cholesterol sulfate compared with wild-type mice, identifying a previously uncharacterized microbiome-transformation of cholesterol and a mechanism for microbiome-dependent contributions to host phenotype. Moreover, identification of a cholesterol-responsive sulfotransferase in Bacteroides suggests diet-dependent mechanisms for altering microbiome-specific cholesterol metabolism. Overall, our work identifies numerous cholesterol-interacting microbes with implications for more precise microbiome-conscious regulation of host cholesterol homeostasis.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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