Cold exposure induces dynamic changes in circulating triacylglycerol species, which is dependent on intracellular lipolysis: A randomized cross-over trial
Straat ME, Jurado-Fasoli L, Ying Z, Nahon KJ, Janssen LGM, Boon MR, Grabner GF, Kooijman S, Zimmermann R, Giera M, Rensen PCN, Martinez-Tellez B
EBioMedicine · 29 citas
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Organización sin fines de lucro
- Fundación Alfonso Martín Escudero
- Organización sin fines de lucro
- European Foundation for the Study of Diabetes
- Gobierno
- ZonMw
- Gobierno
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Gobierno
- Ministerio de Universidades
- Gobierno
- NextGenerationEU
- Gobierno
- Dutch Research Council (NWO)
- Subvenciones
- ZonMw (184.034.019); Nederlandse Organisatie voor Wetenschappelijk Onderzoek (184.034.019)
Según 7 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2022-11-11 · EBioMedicine · vol. 86 · pág. 104349
- Editorial
- Elsevier BV
- Citado
- 35 citas · más que el 93% de estudios similares · 3.0× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 62 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Adipose Tissue and Metabolism · Lipid metabolism and biosynthesis · Lipid metabolism and disorders
- Palabras clave
- cold exposure, lipidome, adipose triglyceride lipase, free fatty acids, hepatic lipid metabolism, thermogenesis, lipid degradation, adipose tissue, serum lipids, mice, human, LC-MS
- MeSH
- adipose tissue, animals, mice, inbred c57bl, humans, mice, lipase, fatty acids, nonesterified, triglycerides, cross-over studies, lipolysis, male, cold temperature
12 autores
- Maaike E. Straat🇳🇱Leiden University Medical Center
- Lucas Jurado‐Fasoli🇪🇸Universidad de Granada
- Zhixiong Ying🇳🇱Leiden University Medical Center
- Kimberly J. Nahon🇳🇱Leiden University Medical Center
- Laura G.M. Janssen🇳🇱Leiden University Medical Center
- Mariëtte R. Boon🇳🇱Leiden University Medical Center
- Gernot F. Grabner🇦🇹University of Graz
- Sander Kooijman🇳🇱Leiden University Medical Center
- Robert Zimmermann🇦🇹University of Graz
- Martin A. Giera🇳🇱Leiden University Medical Center
- Patrick C.N. Rensen🇳🇱 · autor de correspondenciaLeiden University Medical Center
- Borja Martínez‐Téllez🇳🇱Leiden University Medical Center
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
The application of cold exposure has emerged as an approach to enhance whole-body lipid catabolism. The global effect of cold exposure on the lipidome in humans has been reported with mixed results depending on intensity and duration of cold.
Methods
This secondary study was based on data from a previous randomized cross-over trial (ClinicalTrials.gov ID: NCT03012113). We performed sequential lipidomic profiling in serum during 120 min cold exposure of human volunteers. Next, the intracellular lipolysis was blocked in mice (eighteen 10-week-old male wild-type mice C57BL/6J) using a small-molecule inhibitor of adipose triglyceride lipase (ATGL; Atglistatin), and mice were exposed to cold for a similar duration. The quantitative lipidomic profiling was assessed in-depth using the Lipidyzer platform.
Findings
In humans, cold exposure gradually increased circulating free fatty acids reaching a maximum at 60 min, and transiently decreased total triacylglycerols (TAGs) only at 30 min. A broad range of TAG species was initially decreased, in particular unsaturated and polyunsaturated TAG species with ≤5 double bonds, while after 120 min a significant increase was observed for polyunsaturated TAG species with ≥6 double bonds in humans. The mechanistic study in mice revealed that the cold-induced increase in polyunsaturated TAGs was largely prevented by blocking adipose triglyceride lipase.
Interpretation
We interpret these findings as that cold exposure feeds thermogenic tissues with TAG-derived fatty acids for combustion, resulting in a decrease of circulating TAG species, followed by increased hepatic production of polyunsaturated TAG species induced by liberation of free fatty acids stemming from adipose tissue.
Funding
This work was supported by the Netherlands CardioVascular Research Initiative: 'the Dutch Heart Foundation, Dutch Federation of University Medical Centers, the Netherlands Organisation for Health Research and Development and the Royal Netherlands Academy of Sciences' [CVON2017-20 GENIUS-II] to Patrick C.N. Rensen. Borja Martinez-Tellez is supported by individual postdoctoral grant from the Fundación Alfonso Martin Escudero and by a Maria Zambrano fellowship by the Ministerio de Universidades y la Unión Europea - NextGenerationEU (RR_C_2021_04). Lucas Jurado-Fasoli was supported by an individual pre-doctoral grant from the Spanish Ministry of Education (FPU19/01609) and with an Albert Renold Travel Fellowship from the European Foundation for the Study of Diabetes (EFSD). Martin Giera was partially supported by NWO XOmics project #184.034.019.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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