15 N-labeled dietary nitrate supplementation increases human skeletal muscle nitrate concentration and improves muscle torque production
Kadach S, Park JW, Stoyanov Z, Black MI, Vanhatalo A, Burnley M, Walter PJ, Cai H, Schechter AN, Piknova B, Jones AM
Acta physiologica (Oxford, England) · 29 citas
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Marcadores de salud y funcionamiento
- Consumo medido con
- No se indica
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- University of Exeter
- Universidad u hospital
- University of Queensland
- Gobierno
- Biotechnology and Biological Sciences Research Council
- Subvenciones
- Biotechnology and Biological Sciences Research Council (BB/S020632/1); Biotechnology and Biological Sciences Research Council (BB/S020632/1)
Según 3 financiadores declarados.
Publicación
- Publicado
- 2023-01-06 · Acta Physiol (Oxf) · vol. 237 · número 3 · pág. e13924
- Editorial
- Wiley
- Citado
- 35 citas · más que el 99% de estudios similares · 11.0× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 57 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY
- Áreas de investigación
- Cardiovascular and exercise physiology · Nitric Oxide and Endothelin Effects · Pharmacological Effects and Assays
- Palabras clave
- Chemistry, Skeletal muscle, Internal medicine, Endocrinology, Crossover study, Ingestion, Nitric oxide, Bioavailability, Placebo, Animal science, Biochemistry, Medicine, Biology, Pharmacology
- MeSH
- muscle, skeletal, humans, nitrates, nitrites, nitric oxide, nitrogen dioxide, polyesters, cross-over studies, double-blind method, blood pressure, torque, dietary supplements
11 autores
- Stefan Kadach🇬🇧University of Exeter
- Ji Won Park🇺🇸National Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
- Zdravko Stoyanov🇬🇧University of Exeter
- Matthew I. Black🇬🇧University of Exeter
- Anni Vanhatalo🇬🇧University of Exeter
- Mark Burnley🇬🇧Loughborough University
- Peter J. Walter🇺🇸National Institutes of Health
- Hongyi Cai🇺🇸National Institutes of Health
- Alan N. Schechter🇺🇸National Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
- Barbora Piknova🇺🇸National Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
- ANDREW MARK JONES🇬🇧 · autor de correspondenciaUniversity of Exeter
Resumen
El texto original del estudio, en el idioma en que se publicó.
Aim
Dietary nitrate (NO3 - ) supplementation increases nitric oxide bioavailability and can enhance exercise performance. We investigated the distribution and metabolic fate of ingested NO3 - at rest and during exercise with a focus on skeletal muscle.
Methods
In a randomized, crossover study, 10 healthy volunteers consumed 12.8 mmol 15 N-labeled potassium nitrate (K15 NO3 ; NIT) or potassium chloride placebo (PLA). Muscle biopsies were taken at baseline, at 1- and 3-h post-supplement ingestion, and immediately following the completion of 60 maximal intermittent contractions of the knee extensors. Muscle, plasma, saliva, and urine samples were analyzed using chemiluminescence to determine absolute [NO3 - ] and [NO2 - ], and by mass spectrometry to determine the proportion of NO3 - and NO2 - that was 15 N-labeled.
Results
Neither muscle [NO3 - ] nor [NO2 - ] were altered by PLA. Following NIT, muscle [NO3 - ] (but not [NO2 - ]) was elevated at 1-h (from ~35 to 147 nmol/g, p 15 N-labeled. There was a significant reduction in 15 N-labeled muscle [NO3 - ] from pre- to post-exercise. Relative to PLA, mean muscle torque production was ~7% greater during the first 18 contractions following NIT. This improvement in torque was correlated with the pre-exercise 15 N-labeled muscle [NO3 - ] and the magnitude of decline in 15 N-labeled muscle [NO3 - ] during exercise (r = 0.66 and r = 0.62, respectively; p < 0.01).
Conclusion
This study shows, for the first time, that skeletal muscle rapidly takes up dietary NO3 - , the elevated muscle [NO3 - ] following NO3 - ingestion declines during exercise, and muscle NO3 - dynamics are associated with enhanced torque production during maximal intermittent muscle contractions.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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