Early cancer detection using the fluorescent Ashwagandha chitosan nanoparticles combined with near-infrared light diffusion characterization: in vitro study
Abuelmakarem HS, Hamdy O, Sliem MA, El-Azab J, Ahmed WA
Lasers in medical science · 6 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- Cairo University
- Gobierno
- Science and Technology Development Fund
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2023-01-11 · Lasers Med Sci · vol. 38 · número 1 · pág. 37
- Editorial
- Springer Science+Business Media
- Citado
- 14 citas · más que el 87% de estudios similares · 2.1× el promedio de su campo
- Referencias
- 58 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY
- Áreas de investigación
- Optical Imaging and Spectroscopy Techniques · Medical and Biological Ozone Research · Photodynamic Therapy Research Studies
- Palabras clave
- Zeta potential, MTT assay, Fluorescence, Irradiation, Nanoparticle, Materials science, Chitosan, Chemistry, Dynamic light scattering, Biophysics, Analytical Chemistry (journal), Nuclear chemistry, Nanotechnology, In vitro, Chromatography, Biochemistry, Optics, Biology
- MeSH
- humans, colonic neoplasms, chitosan, plant extracts, spectroscopy, fourier transform infrared, coloring agents, nanoparticles
5 autores
- Hala S. Abuelmakarem🇪🇬 · autor de correspondenciaCairo University; El Shorouk Academy; Higher Institute of Engineering
- Omnia Hamdy🇪🇬Cairo University
- Mahmoud A. Sliem🇪🇬🇸🇦Cairo University; Taibah University; Nile University
- Jala M. El-Azab🇪🇬Cairo University
- Wafaa Abdallah Ahmed🇪🇬Cairo University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Early cancer diagnosis through characterizing light propagation and nanotechnology increases the survival rate. The present research is aimed at evaluating the consequence of using natural nanoparticles in cancer therapy and diagnosis. Colon cancer cells were differentiated from the normal cells via investigating light diffusion combined with the fluorescence effect of the Ashwagandha chitosan nanoparticles (Ash C NPs). Ionic gelation technique synthesized the Ash C NPs. High-resolution transmission electron microscope, dynamic light scattering, and zeta potential characterized Ash C NPs. Fourier transform infrared spectroscopy analyzed Ash C NPs, chitosan, and Ashwagandha root water extract. Moreover, the MTT assay evaluated the cytotoxicity of Ash C NPs under the action of near-infrared light (NIR) irradiation. The MTT assay outcomes were statistically analyzed by Bonferroni post hoc multiple two-group comparisons using one-way variance analysis (ANOVA). Based on the Monte-Carlo simulation technique, the spatially resolved steady-state diffusely reflected light from the cancerous and healthy cells is acquired. The diffuse equation reconstructed the optical fluence rate using the finite element technique. The fluorescent effect of the nanoparticles was observed when the cells were irradiated with NIR. The MTT assay revealed a decrease in the cell viability under the action of Ash C NPs with and without laser irradiation. Colon cancer and normal cells were differentiated based on the optical characterization after laser irradiation. The light diffusion equation was successfully resolved for the fluence rate on cells' surfaces showing different normal and cancer cells values. Ash C NPs appeared its fluorescent effect in the presence of NIR laser.
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