Sulforaphane Supplementation Did Not Modulate NRF2 and NF-kB mRNA Expressions in Hemodialysis Patients
Ribeiro M, Cardozo LF, Paiva BR, Baptista BG, Fanton S, Alvarenga L, Lima LS, Britto I, Nakao LS, Fouque D, Ribeiro-Alves M, Mafra D
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation · 9 citas
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
- Consumo medido con
- No se indica
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2023-08-22 · J Ren Nutr · vol. 34 · número 1 · págs. 68–75
- Editorial
- Elsevier BV
- Citado
- 10 citas · más que el 66% de estudios similares · 0.7× el promedio de su campo
- Referencias
- 40 trabajos
- Acceso
- De pago
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Dialysis and Renal Disease Management · Nigella sativa pharmacological applications
- Palabras clave
- sulforaphane, Nrf2, NF-κB, hemodialysis, chronic kidney disease, myrosinase, cruciferous vegetables, food as medicine, oxidative stress, inflammation, lipid peroxidation, malondialdehyde, TNF-α, interleukin-6, dietary supplementation
- MeSH
- humans, isothiocyanates, sulfoxides, nf-kappa b, rna, messenger, renal dialysis, cross-over studies, oxidative stress, dietary supplements, female, nf-e2-related factor 2, renal insufficiency, chronic
12 autores
- Marcia Maria Ribeiro🇧🇷Universidade Federal do Rio de Janeiro
- L. Cardozo🇧🇷Universidade Federal Fluminense
- Bruna R. Paiva🇧🇷Universidade Federal Fluminense
- Beatriz Germer Baptista🇧🇷Universidade Federal Fluminense
- Susane Fanton🇧🇷Universidade Federal Fluminense
- Lívia Alvarenga🇧🇷Universidade Federal do Rio de Janeiro; Universidade Federal Fluminense
- Ligia Soares Lima🇧🇷Universidade Federal do Rio de Janeiro
- Isadora K. Britto🇧🇷Universidade Federal do Rio de Janeiro
- Lia S. Nakao🇧🇷Universidade Federal do Paraná
- Denis P. Fouque🇫🇷Inserm; Université de Lyon; Hôpital Lyon Sud
- Marcelo Ribeiro Alves🇧🇷Fundação Oswaldo Cruz
- Denise Mafra🇧🇷 · autor de correspondenciaUniversidade Federal do Rio de Janeiro; Universidade Federal Fluminense
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Patients with chronic kidney disease (CKD) have reduced expression of erythroid nuclear factor-related factor 2 (NRF2) and increased nuclear factor κB (NF-κB). "Food as medicine" has been proposed as an adjuvant therapeutic alternative in modulating these factors. No studies have investigated the effects of sulforaphane (SFN) in cruciferous vegetables on the expression of these genes in patients with CKD.
Objective
The study aimed to evaluate the effects of SFN on the expression of NRF2 and NF-κB in patients on hemodialysis (HD).
Design and methods
A randomized, double-blind, crossover study was performed on 30 patients on regular HD. Fourteen patients were randomly allocated to the intervention group (1 sachet/day of 2.5 g containing 1% SFN extract with 0.5% myrosinase) and 16 patients to the placebo group (1 sachet/day of 2.5 g containing corn starch colored with chlorophyll) for 2 months. After a washout period of 2 months, the groups were switched. NRF2 and NF-κB mRNA expression was evaluated by real-time quantitative polymerase chain reaction, and tumor necrosis factor alpha and interleukin-6 levels were quantified by enzyme-linked immunosorbent assay. Malondialdehyde was evaluated as a marker of lipid peroxidation.
Results
Twenty-five patients (17 women, 55 [interquartile range = 19] years and 55 [interquartile range = 74] months on HD) completed the study. There was no significant difference concerning the expression of mRNA NRF2 (P = .915) and mRNA NF-κB (P = .806) after supplementation with SFN. There was no difference in pro-inflammatory and oxidative stress biomarkers.
Conclusion
150 μmol of SFN for 2 months had no antioxidant and anti-inflammatory effect in patients with CKD undergoing HD.
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