Six-hour time-restricted feeding inhibits lung cancer progression and reshapes circadian metabolism
Shi D, Fang G, Chen Q, Li J, Ruan X, Lian X
BMC medicine · 31 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Eventos clínicos como enfermedad o muerte
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- Chongqing Postdoctoral Science Foundation
- Gobierno
- National Natural Science Foundation of China
- Gobierno
- China Postdoctoral Science Foundation
- Universidad u hospital
- Chongqing Medical University
- Gobierno
- Chongqing Municipal Education Commission
- Organización sin fines de lucro
- Postdoctoral Research Foundation of China
- Sin clasificar
- Young Scientists Fund
- Subvenciones
- Postdoctoral Research Foundation of China (2021M693755); National Natural Science Foundation of China (8210120502); National Natural Science Foundation of China (81973030); Chongqing Postdoctoral Science Foundation ([2020] 379); Chongqing Medical University (W0085); Young Scientists Fund (8210120502)
Según 7 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2023-11-03 · BMC Med · vol. 21 · número 1 · pág. 417
- Editorial
- BioMed Central
- Citado
- 40 citas · más que el 97% de estudios similares · 5.5× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 61 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Dietary Effects on Health · Circadian rhythm and melatonin · Epigenetics and DNA Methylation
- Palabras clave
- Downregulation and upregulation, Lung cancer, Circadian rhythm, Gene knockdown, Tumor progression, Cancer research, Medicine, Cancer, Autophagy, Cancer cell, Oncogene, Internal medicine, Biology, Cell cycle, Cell culture, Gene, Apoptosis, Biochemistry
- MeSH
- lung, animals, humans, mice, lung neoplasms, disease models, animal, circadian rhythm, intermittent fasting
6 autores
- Dan Shi🇨🇳 · autor de correspondenciaDalian Medical University; Second Affiliated Hospital of Chongqing Medical University; Chongqing Public Health Medical Center; Chongqing Medical University
- Gaofeng Fang🇨🇳Chongqing Public Health Medical Center; Chongqing Medical University
- Qianyao Chen🇨🇳Chongqing Public Health Medical Center; Chongqing Medical University
- Jianling Li🇨🇳Chongqing Public Health Medical Center; Chongqing Medical University
- Xiongzhong Ruan🇨🇳 · autor de correspondenciaDalian Medical University; Second Affiliated Hospital of Chongqing Medical University; Chongqing Medical University
- Xuemei Lian🇨🇳 · autor de correspondenciaDalian Medical University; Second Affiliated Hospital of Chongqing Medical University; Chongqing Public Health Medical Center; Chongqing Medical University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Accumulating evidence has suggested an oncogenic effect of diurnal disruption on cancer progression. To test whether targeting circadian rhythm by dietary strategy suppressed lung cancer progression, we adopted 6-h time-restricted feeding (TRF) paradigm to elucidate whether and how TRF impacts lung cancer progression.
Methods
This study used multiple lung cancer cell lines, two xenograft mouse models, and a chemical-treated mouse lung cancer model. Stable TIM-knockdown and TIM-overexpressing A549 cells were constructed. Cancer behaviors in vitro were determined by colony formation, EdU proliferation, wound healing, transwell migration, flow cytometer, and CCK8 assays. Immunofluorescence, pathology examinations, and targeted metabolomics were also used in tumor cells and tissues. mCherry-GFP-LC3 plasmid was used to detect autophagic flux.
Results
We found for the first time that compared to normal ad libitum feeding, 6-h TRF inhibited lung cancer progression and reprogrammed the rhythms of metabolites or genes involved in glycolysis and the circadian rhythm in tumors. After TRF intervention, only timeless (TIM) gene among five lung cancer-associated clock genes was found to consistently align rhythm of tumor cells to that of tumor tissues. Further, we demonstrated that the anti-tumor effect upon TRF was partially mediated by the rhythmic downregulation of the TIM and the subsequent activation of autophagy. Combining TRF with TIM inhibition further enhanced the anti-tumor effect, comparable to treatment efficacy of chemotherapy in xenograft model.
Conclusions
Six-hour TRF inhibits lung cancer progression and reshapes circadian metabolism, which is partially mediated by the rhythmic downregulation of the TIM and the subsequent upregulation of autophagy.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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