Molecular Insights into the Inhibition of Lipid Accumulation in Hepatocytes by Unique Extracts of Ashwagandha
Li D, Han H, Sun Y, Zhang H, Yoshitomi R, Kaul SC, Wadhwa R
International journal of molecular sciences · 2 citas
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- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Células o muestras de laboratorio
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- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Advanced Industrial Science and Technology
- Gobierno
- AIST (Japan)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2024-11-14 · Int J Mol Sci · vol. 25 · número 22 · pág. 12256
- Editorial
- Multidisciplinary Digital Publishing Institute
- Citado
- 3 citas · más que el 74% de estudios similares · 0.8× el promedio de su campo
- Referencias
- 67 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Liver Disease Diagnosis and Treatment · Drug-Induced Hepatotoxicity and Protection · Phytochemicals and Medicinal Plants
- Palabras clave
- Withaferin A, Withanolide, Lipogenesis, Chemistry, Peroxisome, Steatosis, Biochemistry, Fatty acid synthase, Oxidative stress, Fatty acid, Nonalcoholic fatty liver disease, Fatty liver, Lipid metabolism, Peroxisome proliferator-activated receptor, Pharmacology, Withania somnifera, Biology, Receptor, Internal medicine, Endocrinology, Medicine
- MeSH
- hepatocytes, humans, withania, fatty acids, nonesterified, plant extracts, oxidative stress, lipogenesis, lipid metabolism, withanolides, hep g2 cells, non-alcoholic fatty liver disease
7 autores
- Dongyang Li🇯🇵National Institute of Advanced Industrial Science and Technology
- Hanlin Han🇯🇵University of Tsukuba; National Institute of Advanced Industrial Science and Technology
- Yixin Sun🇯🇵University of Tsukuba; National Institute of Advanced Industrial Science and Technology
- Huayue Zhang🇯🇵University of Tsukuba; National Institute of Advanced Industrial Science and Technology
- Ren Yoshitomi🇯🇵National Institute of Advanced Industrial Science and Technology
- Sunil C. Kaul🇯🇵National Institute of Advanced Industrial Science and Technology
- Renu Wadhwa🇯🇵 · autor de correspondenciaUniversity of Tsukuba; National Institute of Advanced Industrial Science and Technology
Resumen
El texto original del estudio, en el idioma en que se publicó.
We investigated the effect of purified withanolides and extracts derived from Ashwagandha on steatosis, the abnormal accumulation of fat that can lead to non-alcoholic fatty liver disease (NAFLD). Collaborator of ARF (CARF, also known as CDKN2AIP, a protein that regulates hepatic lipid metabolism, fat buildup, and liver damage) was used as an indicator. Six withanolides (Withaferin A, Withanone, Withanolide B, Withanoside IV, Withanoside V, and Withanostraminolide-12 deoxy) reversed the decrease in CARF caused by exposure to free fatty acids (FFAs) in liver-derived cells (HepG2 hepatocytes). After analyzing the effects of these withanolides on CARF mRNA and protein levels, FFA accumulation, protein aggregation, and oxidative and DNA damage stresses, we selected Withaferin A and Withanone for molecular analyses. Using the palmitic-acid-induced fatty acid accumulation stress model in Huh7 cells, we found a significant reduction in the activity of the key regulators of lipogenesis pathways, including sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FASN), and peroxisome proliferator-activated receptors (PPARγ and PPARα). This in vitro study suggests that low, non-toxic doses of Withaferin A, Withanone, or Ashwagandha extracts containing these withanolides possess anti-steatosis and antioxidative-stress properties. Further in vivo and clinical studies are required to investigate the therapeutic potential of these Ashwagandha-derived bioactive ingredients for NAFLD.
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