Ashwagandha (<i>Withania somnifera</i>) Plant Extracts Affect the Cytochrome P450 System and Cytotoxicity of Primary Human Hepatocytes
McDonald KL, Raichura Z, Pondugula SR, Marney L, Yang L, Choi J, Salamat JM, Brandes MS, Neff C, Adams K, Farmer G, Dennis C, Maier CS, Soumyanath A, Arnold RD, Calderón AI
Journal of dietary supplements · 6 citas
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- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institutes of Health
- Gobierno
- National Center for Research Resources
- Gobierno
- National Center for Complementary and Integrative Health
- Gobierno
- National Institutes of Health Office of the Director
- Gobierno
- National Institutes of Health National Center for Complementary and Integrative Health
- Gobierno
- NCCIH NIH HHS
- Gobierno
- National Institutes of Health National Center for Research Resources
- Gobierno
- NIH HHS
- Gobierno
- NCRR NIH HHS
- Subvenciones
- National Center for Complementary and Integrative Health (U19 AT010829); National Center for Research Resources (S10 RR027878); National Center for Complementary and Integrative Health (R03 AT011501); National Institutes of Health (1s10od026922-01)
Según 9 financiadores declarados.
Publicación
- Publicado
- 2025-06-24 · J Diet Suppl · vol. 22 · número 4 · págs. 613–639
- Editorial
- Taylor & Francis
- Citado
- 7 citas · más que el 97% de estudios similares · 7.9× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 48 trabajos
- Acceso
- Acceso abierto (copia en repositorio)
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection
- Palabras clave
- Withania somnifera, Cytotoxicity, Viability assay, Pharmacology, Traditional medicine, Cytochrome P450, Nutraceutical, Biology, Chemistry, Biochemistry, Enzyme, In vitro, Medicine
- MeSH
- cells, cultured, hepatocytes, humans, withania, plant leaves, plant roots, cytochrome p-450 enzyme system, cytochrome p-450 cyp1a2, plant extracts, cell survival, herb-drug interactions, cytochrome p-450 cyp3a, hep g2 cells, cytochrome p-450 cyp2b6
16 autores
- Kelli L. McDonald🇺🇸Auburn University
- Zarna Raichura🇺🇸Auburn University
- Satyanarayana R. Pondugula🇺🇸Auburn University
- Luke C. Marney🇺🇸Oregon State University; Oregon Health & Science University
- Liping Yang🇺🇸Oregon State University; Oregon Health & Science University
- Jae‐Woo Choi🇺🇸Oregon State University; Oregon Health & Science University
- Julia M. Salamat🇺🇸Auburn University
- Mikah S. Brandes🇺🇸Oregon Health & Science University
- Cody J. Neff🇺🇸Oregon Health & Science University
- Keila Adams🇺🇸Auburn University
- Gracie Farmer🇺🇸Auburn University
- Catherine Dennis🇺🇸Auburn University
- Claudia S. Maier🇺🇸Oregon State University; Oregon Health & Science University
- Amala Soumyanath🇺🇸Oregon Health & Science University
- Robert D. Arnold🇺🇸Auburn University
- Ángela I. Calderón🇺🇸 · autor de correspondenciaAuburn University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Ashwagandha (Withania somnifera [L] Dunal) is emerging as one of the top 10 botanicals in the market due to its claimed health benefits as an adaptogen. Alongside its popularity, the lack of standardization in botanical dietary supplements and potential interactions with prescription drugs raise safety concerns. This study aimed to investigate the potential of ashwagandha extracts to induce cytochrome P450 (P450) enzymes, which are critical for drug metabolism and implicated in botanical-drug interactions. A sandwich-cultured in vitro assay using primary human hepatocytes was utilized to evaluate the induction potential of aqueous and 70% ethanolic extracts of ashwagandha roots and leaves on CYP3A4, CYP2B6, and CYP1A2-enzymes commonly involved in drug metabolism. Unlike prior studies that focused on root extracts or used HepG2 cell lines, this research included both root and leaf plant extracts, with the latter being assessed for the first time. Our approach using primary hepatocytes enhances clinical relevance compared to other in vitro models (such as HepG2 cell lines). Additionally, cytotoxicity was examined using CellTitre-Glo Luminescent Assay, measuring cell viability at three different incubation times (24, 48, and 72 hours) to assess the potential cytotoxic effect of the ashwagandha plant extracts. Our findings revealed that 70% ethanol ashwagandha root extracts (WSR-2) modulated CYP3A4 enzyme expression and activity. Preliminary studies on cytotoxicity indicated dose- and time-dependent impact on hepatocyte viability with a high concentration of 70% ethanol ashwagandha leaf extracts (WSL-2). The study highlights the importance of understanding ashwagandha's impact on P450-based drug metabolism and its safe co-administration with prescribed drugs.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
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